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anti cd9 mouse polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech anti cd9 mouse polyclonal antibody
    Anti Cd9 Mouse Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 627 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd9+mouse+antibody/CD9+Antibody/pmc12830253-99-15-19
    Average 96 stars, based on 627 article reviews
    anti cd9 mouse polyclonal antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction.
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232- 1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232-1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Blocking Assay:

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction.
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232- 1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232-1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Proximity Ligation Assay:

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction.
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232- 1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232-1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Ligation:

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction.
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232- 1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232-1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Amplification:

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction.
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232- 1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    Article Title: CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction
    Article Snippet: .. In brief, then, slides were fixed and permeabilized, and incubated with blocking solution for 30 min at 37 °C, primary antibody CD44 Rabbit antibody (Proteintech, 15675-1-AP, 1:200), FGFR2 Mouse antibody (Abcam, ab58201, 1:50), LaminA/C Mouse antibody (CST, 4777, 1:100) and CD9 Mouse antibody (Proteintech, 60232-1-Ig, 1:200) overnight at 4 °C, PLA probe solutions for 60 min at 37 °C, Ligation-Ligase solution for 30 min at 37 °C, and Amplification-Polymerase solution for 100 min at 37 °C, sequentially. ..

    other:

    Article Title: Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis
    Article Snippet: CD63 rabbit antibody (25682-1-AP), CD81 mouse antibody (66866-1-Ig), and CD9 mouse antibody (60232-1-Ig) were purchased from Proteintech (Rosemont, IL).



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    EV marker profiling by immunogold TEM and europium nanoparticle‐based immunoassays. (A) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against <t>CD9.</t> (B) Quantification of CD9 expression on cell line‐derived EVs using europium nanoparticle‐based assays. The SEC‐isolated pooled fractions F1‐F3 were analysed for relative tetraspanin expression in different cell types. Graphs present the average of 3 individual experiment values; error bars represent SEM values. (C) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against CD81. (D) CD81 expression quantification on cell line‐derived EVs using europium nanoparticle‐based assays. CD9 was measured via biotin streptavidin fluorescence immunoassay (BSFIA), while CD81 were assessed by time‐resolved immunofluorometric assay (TRFIA). Y‐axis in panels B is shown on a log10 scale. All data represent the mean from three independent experiments. Scale bars in TEM images = 100 nm.
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    EV marker profiling by immunogold TEM and europium nanoparticle‐based immunoassays. (A) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against <t>CD9.</t> (B) Quantification of CD9 expression on cell line‐derived EVs using europium nanoparticle‐based assays. The SEC‐isolated pooled fractions F1‐F3 were analysed for relative tetraspanin expression in different cell types. Graphs present the average of 3 individual experiment values; error bars represent SEM values. (C) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against CD81. (D) CD81 expression quantification on cell line‐derived EVs using europium nanoparticle‐based assays. CD9 was measured via biotin streptavidin fluorescence immunoassay (BSFIA), while CD81 were assessed by time‐resolved immunofluorometric assay (TRFIA). Y‐axis in panels B is shown on a log10 scale. All data represent the mean from three independent experiments. Scale bars in TEM images = 100 nm.
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    Miltenyi Biotec cd9 antibody, anti-mouse
    EV marker profiling by immunogold TEM and europium nanoparticle‐based immunoassays. (A) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against <t>CD9.</t> (B) Quantification of CD9 expression on cell line‐derived EVs using europium nanoparticle‐based assays. The SEC‐isolated pooled fractions F1‐F3 were analysed for relative tetraspanin expression in different cell types. Graphs present the average of 3 individual experiment values; error bars represent SEM values. (C) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against CD81. (D) CD81 expression quantification on cell line‐derived EVs using europium nanoparticle‐based assays. CD9 was measured via biotin streptavidin fluorescence immunoassay (BSFIA), while CD81 were assessed by time‐resolved immunofluorometric assay (TRFIA). Y‐axis in panels B is shown on a log10 scale. All data represent the mean from three independent experiments. Scale bars in TEM images = 100 nm.
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    Image Search Results


    EV marker profiling by immunogold TEM and europium nanoparticle‐based immunoassays. (A) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against CD9. (B) Quantification of CD9 expression on cell line‐derived EVs using europium nanoparticle‐based assays. The SEC‐isolated pooled fractions F1‐F3 were analysed for relative tetraspanin expression in different cell types. Graphs present the average of 3 individual experiment values; error bars represent SEM values. (C) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against CD81. (D) CD81 expression quantification on cell line‐derived EVs using europium nanoparticle‐based assays. CD9 was measured via biotin streptavidin fluorescence immunoassay (BSFIA), while CD81 were assessed by time‐resolved immunofluorometric assay (TRFIA). Y‐axis in panels B is shown on a log10 scale. All data represent the mean from three independent experiments. Scale bars in TEM images = 100 nm.

    Journal: Journal of Extracellular Biology

    Article Title: Molecular Characterization of Extracellular Vesicles From Human B Cell Lymphomas: Methodological Comparison to Vesicles From Patient Serum

    doi: 10.1002/jex2.70107

    Figure Lengend Snippet: EV marker profiling by immunogold TEM and europium nanoparticle‐based immunoassays. (A) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against CD9. (B) Quantification of CD9 expression on cell line‐derived EVs using europium nanoparticle‐based assays. The SEC‐isolated pooled fractions F1‐F3 were analysed for relative tetraspanin expression in different cell types. Graphs present the average of 3 individual experiment values; error bars represent SEM values. (C) Immunogold TEM images of EVs isolated from ABC‐type (U‐2, Ri) and GCB‐type (S‐4, O‐7) DLBCL cell lines, labeled with antibodies against CD81. (D) CD81 expression quantification on cell line‐derived EVs using europium nanoparticle‐based assays. CD9 was measured via biotin streptavidin fluorescence immunoassay (BSFIA), while CD81 were assessed by time‐resolved immunofluorometric assay (TRFIA). Y‐axis in panels B is shown on a log10 scale. All data represent the mean from three independent experiments. Scale bars in TEM images = 100 nm.

    Article Snippet: Mouse CD9 antibody , Proteintech ab , MAB1880 , WB iEM, IF.

    Techniques: Marker, Isolation, Labeling, Expressing, Derivative Assay, Fluorescence

    Size‐dependent analysis of CD9 expression in EVs from DLBCL cell lines using immunogold TEM. (A) Representative TEM image of CD9‐labeled EVs from U‐2 (ABC subtype) showing surface localization of gold particles (red arrows). EVs larger than 100 nm are marked in magenta; vesicles and particles smaller than 100 nm are marked in cyan. (B) Average percentage of CD9‐positive EVs across all size ranges in four DLBCL cell lines (U‐2, Ri, S‐4, O‐7). (C, D) Size‐stratified analysis showing the proportion of CD9‐positive EVs in the <100 nm (C) and >100 nm (D) EV subpopulations. (E, F) Total number of EVs measured per group for the corresponding size categories: <100 nm (E) and >100 nm (F). ABC‐type EVs (U‐2, Ri) showed stronger CD9 positivity, particularly in the >100 nm population. Data represent the mean from three independent experiments (total n = 300 n = 100 per experiment). Scale bar = 100 nm.

    Journal: Journal of Extracellular Biology

    Article Title: Molecular Characterization of Extracellular Vesicles From Human B Cell Lymphomas: Methodological Comparison to Vesicles From Patient Serum

    doi: 10.1002/jex2.70107

    Figure Lengend Snippet: Size‐dependent analysis of CD9 expression in EVs from DLBCL cell lines using immunogold TEM. (A) Representative TEM image of CD9‐labeled EVs from U‐2 (ABC subtype) showing surface localization of gold particles (red arrows). EVs larger than 100 nm are marked in magenta; vesicles and particles smaller than 100 nm are marked in cyan. (B) Average percentage of CD9‐positive EVs across all size ranges in four DLBCL cell lines (U‐2, Ri, S‐4, O‐7). (C, D) Size‐stratified analysis showing the proportion of CD9‐positive EVs in the <100 nm (C) and >100 nm (D) EV subpopulations. (E, F) Total number of EVs measured per group for the corresponding size categories: <100 nm (E) and >100 nm (F). ABC‐type EVs (U‐2, Ri) showed stronger CD9 positivity, particularly in the >100 nm population. Data represent the mean from three independent experiments (total n = 300 n = 100 per experiment). Scale bar = 100 nm.

    Article Snippet: Mouse CD9 antibody , Proteintech ab , MAB1880 , WB iEM, IF.

    Techniques: Expressing, Labeling